Differences in gene expression and cytokine production by crystalline vs. amorphous silica in human lung epithelial cells.

ABSTRACT:

Background

Exposure to respirable crystalline silica particles, as opposed to amorphous silica, is associated with lung inflammation, pulmonary fibrosis (silicosis), and potentially with lung cancer. We used Affymetrix/GeneSifter microarray analysis to determine whether gene expression profiles differed in a human bronchial epithelial cell line (BEAS 2B) exposed to cristobalite vs. amorphous silica particles at non-toxic and equal surface areas (75 and 150 × 106μm2/cm2). Bio-Plex analysis was also used to determine profiles of secreted cytokines and chemokines in response to both particles. Finally, primary human bronchial epithelial cells (NHBE) were used to comparatively assess silica particle-induced alterations in gene expression.

Results

Microarray analysis at 24 hours

SUBMITTER: Perkins TN 

PROVIDER: S-EPMC3337246 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature