Production and proteomic characterisation of purified protein derivative from Mycobacterium avium subsp. paratuberculosis.

ABSTRACT:

Background

Effective diagnosis of Johne's disease (JD), particularly at the stage of early subclinical infection, remains one of the greatest challenges for the control of JD worldwide. The IFN-γ test of cell mediated immunity is currently one of the most suitable diagnostics for subclinical infections, however a major limitation of this test is the lack of a standardised purified protein derivative (PPD) antigen (also referred to as Johnin PPD or PPDj). While attempting to replace PPDj with more specific individual antigens is an attractive proposition, bacterial culture derived PPDj remains the most effective antigen preparation for the diagnosis of subclinical JD. It may be possible to increase the reproducibility and specificity of PPDj preparations by further characterising an

SUBMITTER: Wynne JW 

PROVIDER: S-EPMC3337294 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature