Sm protein down-regulation leads to defects in nuclear pore complex disassembly and distribution in C. elegans embryos.

ABSTRACT: Nuclear pore complexes (NPCs) are large macromolecular structures embedded in the nuclear envelope (NE), where they facilitate exchange of molecules between the cytoplasm and the nucleoplasm. In most cell types, NPCs are evenly distributed around the NE. However, the mechanisms dictating NPC distribution are largely unknown. Here, we used the model organism Caenorhabditis elegans to identify genes that affect NPC distribution during early embryonic divisions. We found that down-regulation of the Sm proteins, which are core components of the spliceosome, but not down-regulation of other splicing factors, led to clustering of NPCs. Down-regulation of Sm proteins also led to incomplete disassembly of NPCs during mitosis, but had no effect on lamina disassembly, suggesting that the defect in N

SUBMITTER: Joseph-Strauss D 

PROVIDER: S-EPMC3337357 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature