Universal quantification of structurally diverse natural products using an evaporative light scattering detector.

ABSTRACT: A lack of good methods for absolute quantification of natural products has limited the accuracy of high-throughput screening. Many currently used methods for quantification are either too slow or not amenable to the structural diversity of natural products. Recent developments in low-temperature evaporative light scattering detectors (ELSD-LT) have overcome several historical limitations of ELSDs, including analyte decomposition and low sensitivity. Primarily, ELSDs have been used for relative quantification and detection of compounds that lack a UV chromophore. In this study, we employ an ELSD-LT for absolute quantification of natural products. Calibration curves were constructed using a weighted least-squares analysis for a diverse set of natural products and other compounds. An average

SUBMITTER: Adnani N 

PROVIDER: S-EPMC3338159 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature