Role of Mediator in regulating Pol II elongation and nucleosome displacement in Saccharomyces cerevisiae.

ABSTRACT: Mediator is a modular multisubunit complex that functions as a critical coregulator of RNA polymerase II (Pol II) transcription. While it is well accepted that Mediator plays important roles in the assembly and function of the preinitiation complex (PIC), less is known of its potential roles in regulating downstream steps of the transcription cycle. Here we use a combination of genetic and molecular approaches to investigate Mediator regulation of Pol II elongation in the model eukaryote, Saccharomyces cerevisiae. We find that ewe (expression without heat shock element) mutations in conserved Mediator subunits Med7, Med14, Med19, and Med21-all located within or adjacent to the middle module-severely diminish heat-shock-induced expression of the Hsf1-regulated HSP82 gene. Interestingly, the

SUBMITTER: Kremer SB 

PROVIDER: S-EPMC3338273 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature