RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues.

ABSTRACT: In situ analysis of biomarkers is highly desirable in molecular pathology because it allows the examination of biomarker status within the histopathological context of clinical specimens. Immunohistochemistry and DNA in situ hybridization (ISH) are widely used in clinical settings to assess protein and DNA biomarkers, respectively, but clinical use of in situ RNA analysis is rare. This disparity is especially notable when considering the abundance of RNA biomarkers discovered through whole-genome expression profiling. This is largely due to the high degree of technical complexity and insufficient sensitivity and specificity of current RNA ISH techniques. Here, we describe RNAscope, a novel RNA ISH technology with a unique probe design strategy that allows simultaneous signal amplification

SUBMITTER: Wang F 

PROVIDER: S-EPMC3338343 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature