Nucleosomes correlate with in vivo progression pattern of de novo methylation of p16 CpG islands in human gastric carcinogenesis.

ABSTRACT:

Background

The exact relationship between nucleosome positioning and methylation of CpG islands in human pathogenesis is unknown.

Methodology/principal findings

In the present study, we characterized the nucleosome position within the p16 CpG island and established a seeding methylation-specific PCR (sMSP) assay based on bisulfite modification to enrich the p16 alleles containing methylated-CpG at the methylation "seeding" sites within its intron-1 in gastric carcinogenesis. The sMSP-positive rate in primary gastric carcinoma (GC) samples (36/40) was significantly higher than that observed in gastritis (19/45) or normal samples (7/13) (P<0.01). Extensive clone sequencing of these sMSP products showed that the density of methylated-CpGs in p16 CpG islands increased gradually

SUBMITTER: Lu ZM 

PROVIDER: S-EPMC3338478 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature