A ranking system for reference libraries of DNA barcodes: application to marine fish species from Portugal.

ABSTRACT:

Background

The increasing availability of reference libraries of DNA barcodes (RLDB) offers the opportunity to the screen the level of consistency in DNA barcode data among libraries, in order to detect possible disagreements generated from taxonomic uncertainty or operational shortcomings. We propose a ranking system to attribute a confidence level to species identifications associated with DNA barcode records from a RLDB. Here we apply the proposed ranking system to a newly generated RLDB for marine fish of Portugal.

Methodology/principal findings

Specimens (n = 659) representing 102 marine fish species were collected along the continental shelf of Portugal, morphologically identified and archived in a museum collection. Samples were sequenced at the barcode region of the

SUBMITTER: Costa FO 

PROVIDER: S-EPMC3338485 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature