Multicolour single molecule imaging in cells with near infra-red dyes.

ABSTRACT:

Background

The autofluorescence background of biological samples impedes the detection of single molecules when imaging. The most common method of reducing the background is to use evanescent field excitation, which is incompatible with imaging beyond the surface of biological samples. An alternative would be to use probes that can be excited in the near infra-red region of the spectrum, where autofluorescence is low. Such probes could also increase the number of labels that can be imaged in multicolour single molecule microscopes. Despite being widely used in ensemble imaging, there is a currently a shortage of information available for selecting appropriate commercial near infra-red dyes for single molecule work. It is therefore important to characterise available near infra-red

SUBMITTER: Tynan CJ 

PROVIDER: S-EPMC3338497 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature