Linking yeast Gcn5p catalytic function and gene regulation using a quantitative, graded dominant mutant approach.

ABSTRACT: Establishing causative links between protein functional domains and global gene regulation is critical for advancements in genetics, biotechnology, disease treatment, and systems biology. This task is challenging for multifunctional proteins when relying on traditional approaches such as gene deletions since they remove all domains simultaneously. Here, we describe a novel approach to extract quantitative, causative links by modulating the expression of a dominant mutant allele to create a function-specific competitive inhibition. Using the yeast histone acetyltransferase Gcn5p as a case study, we demonstrate the utility of this approach and (1) find evidence that Gcn5p is more involved in cell-wide gene repression, instead of the accepted gene activation associated with HATs, (2) identify

SUBMITTER: Lanza AM 

PROVIDER: S-EPMC3338614 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature