The airway microbiota in cystic fibrosis: a complex fungal and bacterial community--implications for therapeutic management.

ABSTRACT:

Background

Given the polymicrobial nature of pulmonary infections in patients with cystic fibrosis (CF), it is essential to enhance our knowledge on the composition of the microbial community to improve patient management. In this study, we developed a pyrosequencing approach to extensively explore the diversity and dynamics of fungal and prokaryotic populations in CF lower airways.

Methodology and principal findings

Fungi and bacteria diversity in eight sputum samples collected from four adult CF patients was investigated using conventional microbiological culturing and high-throughput pyrosequencing approach targeting the ITS2 locus and the 16S rDNA gene. The unveiled microbial community structure was compared to the clinical profile of the CF patients. Pyrosequencing conf

SUBMITTER: Delhaes L 

PROVIDER: S-EPMC3338676 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature