Transcriptome sequencing revealed significant alteration of cortical promoter usage and splicing in schizophrenia.

ABSTRACT:

Background

While hybridization based analysis of the cortical transcriptome has provided important insight into the neuropathology of schizophrenia, it represents a restricted view of disease-associated gene activity based on predetermined probes. By contrast, sequencing technology can provide un-biased analysis of transcription at nucleotide resolution. Here we use this approach to investigate schizophrenia-associated cortical gene expression.

Methodology/principal findings

The data was generated from 76 bp reads of RNA-Seq, aligned to the reference genome and assembled into transcripts for quantification of exons, splice variants and alternative promoters in postmortem superior temporal gyrus (STG/BA22) from 9 male subjects with schizophrenia and 9 matched non-psychiatric

SUBMITTER: Wu JQ 

PROVIDER: S-EPMC3338678 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature