Generation and analysis of a mouse intestinal metatranscriptome through Illumina based RNA-sequencing.

ABSTRACT: With the advent of high through-put sequencing (HTS), the emerging science of metagenomics is transforming our understanding of the relationships of microbial communities with their environments. While metagenomics aims to catalogue the genes present in a sample through assessing which genes are actively expressed, metatranscriptomics can provide a mechanistic understanding of community inter-relationships. To achieve these goals, several challenges need to be addressed from sample preparation to sequence processing, statistical analysis and functional annotation. Here we use an inbred non-obese diabetic (NOD) mouse model in which germ-free animals were colonized with a defined mixture of eight commensal bacteria, to explore methods of RNA extraction and to develop a pipeline for the gener

SUBMITTER: Xiong X 

PROVIDER: S-EPMC3338770 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature