The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria.

ABSTRACT: Protein synthesis by ribosomes takes place on a linear substrate but at non-uniform speeds. Transient pausing of ribosomes can affect a variety of co-translational processes, including protein targeting and folding. These pauses are influenced by the sequence of the messenger RNA. Thus, redundancy in the genetic code allows the same protein to be translated at different rates. However, our knowledge of both the position and the mechanism of translational pausing in vivo is highly limited. Here we present a genome-wide analysis of translational pausing in bacteria by ribosome profiling--deep sequencing of ribosome-protected mRNA fragments. This approach enables the high-resolution measurement of ribosome density profiles along most transcripts at unperturbed, endogenous expression levels. U

SUBMITTER: Li GW 

PROVIDER: S-EPMC3338875 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature