"Boom" and "Bust" cycles in virus growth suggest multiple selective forces in influenza a evolution.

ABSTRACT:

Background

Influenza A virus evolution in humans is driven at least in part by mutations allowing the virus to escape antibody neutralization. Little is known about the evolution of influenza in birds, a major reservoir of influenza A.

Methods

Neutralizing polyclonal antiserum was raised in chicken against reassortant influenza virus, CalX, bearing the hemagglutinin (HA) and neuraminidase (NA) of A/California/7/2004 [H3N2]. CalX was serially passaged in the presence of anti-CalX polyclonal IgY to derive viruses capable of growth in the presence of antibody.

Results

Polyclonal chicken antibody neutralized both HA activity and infection by CalX, but had no effect on a strain bearing an earlier human H3 and an irrelevant neuraminidase (A/Memphis/71-Bellamy/42 [H3N1]). Su

SUBMITTER: Thangavel RR 

PROVIDER: S-EPMC3339368 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature