Y265C DNA polymerase beta knockin mice survive past birth and accumulate base excision repair intermediate substrates.

ABSTRACT: DNA is susceptible to damage by a wide variety of chemical agents that are generated either as byproducts of cellular metabolism or exposure to man-made and harmful environments. Therefore, to maintain genomic integrity, having reliable DNA repair systems is important. DNA polymerase β is known to be a key player in the base excision repair pathway, and mice devoid of DNA polymerase beta do not live beyond a few hours after birth. In this study, we characterized mice harboring an impaired pol β variant. This Y265C pol β variant exhibits slow DNA polymerase activity but WT lyase activity and has been shown to be a mutator polymerase. Mice expressing Y265C pol β are born at normal Mendelian ratios. However, they are small, and 60% die within a few hours after birth. Slow proliferation and si

SUBMITTER: Senejani AG 

PROVIDER: S-EPMC3340078 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature