DnaJ (Hsp40 protein) binding to folded substrate impacts KplE1 prophage excision efficiency.

ABSTRACT: Temperate phages mediate gene transfer and can modify the properties of their host organisms through the acquisition of novel genes, a process called lysogeny. The KplE1 prophage is one of the 10 prophage regions in Escherichia coli K12 MG1655. KplE1 is defective for lysis but fully competent for site-specific recombination. The TorI recombination directionality factor is strictly required for prophage excision from the host genome. We have previously shown that DnaJ promotes KplE1 excision by increasing the affinity of TorI for its site-specific recombination DNA target. Here, we provide evidence of a direct association between TorI and DnaJ using in vitro cross-linking assays and limited proteolysis experiments that show that this interaction allows both proteins to be transiently protec

SUBMITTER: Puvirajesinghe TM 

PROVIDER: S-EPMC3340152 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature