Massively parallel haplotyping on microscopic beads for the high-throughput phase analysis of single molecules.

ABSTRACT: In spite of the many advances in haplotyping methods, it is still very difficult to characterize rare haplotypes in tissues and different environmental samples or to accurately assess the haplotype diversity in large mixtures. This would require a haplotyping method capable of analyzing the phase of single molecules with an unprecedented throughput. Here we describe such a haplotyping method capable of analyzing in parallel hundreds of thousands single molecules in one experiment. In this method, multiple PCR reactions amplify different polymorphic regions of a single DNA molecule on a magnetic bead compartmentalized in an emulsion drop. The allelic states of the amplified polymorphisms are identified with fluorescently labeled probes that are then decoded from images taken of the arrayed

SUBMITTER: Boulanger J 

PROVIDER: S-EPMC3340404 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature