The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.

ABSTRACT:

Background

The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and others have shown that Bxb1 has catalytic activity in various eukaryotic species including Nicotiana tabacum, Schizosaccharomyces pombe, insects and mammalian cells.

Results

In this work, the Bxb1 recombinase gene was transformed and constitutively expressed in Arabidopsis thaliana plants harboring a chromosomally integrated attP and attB-flanked target sequence. The Bxb1 recombinase successfully excised the target sequence in a conservative manner and the resulting recombination event was heritably transmitted to subsequent generations in the absence of the recombinase transgene. In addition, we al

SUBMITTER: Thomson JG 

PROVIDER: S-EPMC3341217 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature