Functional evidence of multidrug resistance transporters (MDR) in rodent olfactory epithelium.

ABSTRACT:

Background

P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP1) are membrane transporter proteins which function as efflux pumps at cell membranes and are considered to exert a protective function against the entry of xenobiotics. While evidence for Pgp and MRP transporter activity is reported for olfactory tissue, their possible interaction and participation in the olfactory response has not been investigated.

Principal findings

Functional activity of putative MDR transporters was assessed by means of the fluorometric calcein acetoxymethyl ester (calcein-AM) accumulation assay on acute rat and mouse olfactory tissue slices. Calcein-AM uptake was measured as fluorescence intensity changes in the presence of Pgp or MRP specific inhibitors. Epifluorescence m

SUBMITTER: Molinas A 

PROVIDER: S-EPMC3341370 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature