Reference genes for real-time PCR quantification of microRNAs and messenger RNAs in rat models of hepatotoxicity.

ABSTRACT: Hepatotoxicity is associated with major changes in liver gene expression induced by xenobiotic exposure. Understanding the underlying mechanisms is critical for its clinical diagnosis and treatment. MicroRNAs are key regulators of gene expression that control mRNA stability and translation, during normal development and pathology. The canonical technique to measure gene transcript levels is Real-Time qPCR, which has been successfully modified to determine the levels of microRNAs as well. However, in order to obtain accurate data in a multi-step method like RT-qPCR, the normalization with endogenous, stably expressed reference genes is mandatory. Since the expression stability of candidate reference genes varies greatly depending on experimental factors, the aim of our study was to identify

SUBMITTER: Lardizabal MN 

PROVIDER: S-EPMC3341372 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature