Intermedin stabilized endothelial barrier function and attenuated ventilator-induced lung injury in mice.

ABSTRACT:

Background

Even protective ventilation may aggravate or induce lung failure, particularly in preinjured lungs. Thus, new adjuvant pharmacologic strategies are needed to minimize ventilator-induced lung injury (VILI). Intermedin/Adrenomedullin-2 (IMD) stabilized pulmonary endothelial barrier function in vitro. We hypothesized that IMD may attenuate VILI-associated lung permeability in vivo.

Methodology/principal findings

Human pulmonary microvascular endothelial cell (HPMVEC) monolayers were incubated with IMD, and transcellular electrical resistance was measured to quantify endothelial barrier function. Expression and localization of endogenous pulmonary IMD, and its receptor complexes composed of calcitonin receptor-like receptor (CRLR) and receptor activity-modifying prote

SUBMITTER: Muller-Redetzky HC 

PROVIDER: S-EPMC3341380 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature