Microfluidic flow cytometer for quantifying photobleaching of fluorescent proteins in cells.

ABSTRACT: Traditional flow cytometers are capable of rapid cellular assays on the basis of fluorescence intensity and light scatter. Microfluidic flow cytometers have largely followed the same path of technological development as their traditional counterparts; however, the significantly smaller transport distance and resulting lower cell speeds in microchannels provides for the opportunity to detect novel spectroscopic signatures based on multiple, nontemporally coincident excitation beams. Here, we characterize the design and operation of a cytometer with a three-beam, probe/bleach/probe geometry, employing HeLa suspension cells expressing fluorescent proteins. The data collection rate exceeds 20 cells/s under a range of beam intensities (5 kW to 179 kW/cm(2)). The measured percent photobleaching

SUBMITTER: Lubbeck JL 

PROVIDER: S-EPMC3341488 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature