Specific tagging of the egress-related osmiophilic bodies in the gametocytes of Plasmodium falciparum.

ABSTRACT:

Background

Gametocytes, the blood stages responsible for Plasmodium falciparum transmission, contain electron dense organelles, traditionally named osmiophilic bodies, that are believed to be involved in gamete egress from the host cell. In order to provide novel tools in the cellular and molecular studies of osmiophilic body biology, a P. falciparum transgenic line in which these organelles are specifically marked by a reporter protein was produced and characterized.

Methodology

A P. falciparum transgenic line expressing an 80-residue N-terminal fragment of the osmiophilic body protein Pfg377 fused to the reporter protein DsRed, under the control of pfg377 upstream and downstream regulatory regions, was produced.

Results

The transgenic fusion protein is expressed at

SUBMITTER: Sannella AR 

PROVIDER: S-EPMC3342164 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature