Long lasting local and systemic inflammation after cerebral hypoxic ischemia in newborn mice.

ABSTRACT:

Background

Hypoxic ischemia (HI) is an important cause of neonatal brain injury and subsequent inflammation affects neurological outcome. In this study we performed investigations of systemic and local activation states of inflammatory cells from innate and adaptive immunity at different time points after neonatal HI brain injury in mice.

Methodology/principal findings

We developed a multiplex flow cytometry based method combined with immunohistochemistry to investigate cellular immune responses in the brain 24 h to 7 months after HI brain injury. In addition, functional studies of ex vivo splenocytes after cerebral hypoxic ischemia were performed. Both central and peripheral activation of CD11b(+) and CD11c(+) antigen presenting cells were seen with expression of the costim

SUBMITTER: Winerdal M 

PROVIDER: S-EPMC3342175 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature