Proteomic characterisation of endoplasmic reticulum-derived protein bodies in tobacco leaves.

ABSTRACT:

Background

The N-terminal proline-rich domain (Zera) of the maize storage protein γ-zein, is able to induce the formation of endoplasmic reticulum (ER)-derived protein bodies (PBs) when fused to proteins of interest. This encapsulation enables a recombinant fused protein to escape from degradation and facilitates its recovery from plant biomass by gradient purification. The aim of the present work was to evaluate if induced PBs encapsulate additional proteins jointly with the recombinant protein. The exhaustive analysis of protein composition of PBs is expected to facilitate a better understanding of PB formation and the optimization of recombinant protein purification approaches from these organelles.

Results

We analysed the proteome of PBs induced in Nicotiana benthamiana

SUBMITTER: Joseph M 

PROVIDER: S-EPMC3342223 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature