Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication.

ABSTRACT:

Aim

To investigate the methylation status of secreted protein acidic and rich in cysteine (SPARC) in human hepatocellular carcinoma (HCC) and evaluate its clinical implication.

Methods

The methylation status of SPARC was analyzed in one HCC cell line (SMMC-7721) and 60 pairs of HCC and corresponding nontumorous tissues by methylation-specific polymerase chain reaction and bisulfite sequencing. The expression of SPARC mRNA and protein were examined by reverse transcription polymerase chain reaction and immunohistochemistry, respectively. The correlations between the methylation status and the gene expression, the clinicopathological parameters, as well as the prognosis after surgery were analyzed.

Results

In the SMMC-7721 cell line, the loss of SPARC expression was cor

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3342602 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature