RNA interference is responsible for reduction of transgene expression after Sleeping Beauty transposase mediated somatic integration.

ABSTRACT:

Background

Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer. For the Sleeping Beauty (SB) transposase system it was demonstrated that convergent transcription driven by the SB transposase inverted repeats (IRs) in eukaryotic cells occurs after somatic integration. This could lead to formation of double-stranded RNAs potentially presenting targets for the RNA interference (RNAi) machinery and subsequently resulting into silencing of the transgene. Therefore, we aimed at investigating transgene expression upon transposition under RNA interference knockdown conditions.

Principal findings

To establish RNAi knockdown cell lines we took advantage of the P19

SUBMITTER: Rauschhuber C 

PROVIDER: S-EPMC3343047 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature