Identification of novel low-dose bisphenol a targets in human foreskin fibroblast cells derived from hypospadias patients.

ABSTRACT:

Background/purpose

The effect of low-dose bisphenol A (BPA) exposure on human reproductive health is still controversial. To better understand the molecular basis of the effect of BPA on human reproductive health, a genome-wide screen was performed using human foreskin fibroblast cells (hFFCs) derived from child hypospadias (HS) patients to identify novel targets of low-dose BPA exposure.

Methodology/principal findings

Gene expression profiles of hFFCs were measured after exposure to 10 nM BPA, 0.01 nM 17β-estradiol (E2) or 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) for 24 h. Differentially expressed genes were identified using an unpaired Student's t test with P value cut off at 0.05 and fold change of more than 1.2. These genes were selected for network generation and

SUBMITTER: Qin XY 

PROVIDER: S-EPMC3344929 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature