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How ubiquitin unfolds after transfer into the gas phase.


ABSTRACT: The structural evolution of ubiquitin after transfer into the gas phase was studied by electron capture dissociation. Site-specific fragment yields show that ubiquitin's solution fold is overall unstable in the gas phase, but unfolding caused by loss of solvent is slowest in regions stabilized by salt bridges.

SUBMITTER: Skinner OS 

PROVIDER: S-EPMC3345118 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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How ubiquitin unfolds after transfer into the gas phase.

Skinner Owen S OS   McLafferty Fred W FW   Breuker Kathrin K  

Journal of the American Society for Mass Spectrometry 20120403 6


The structural evolution of ubiquitin after transfer into the gas phase was studied by electron capture dissociation. Site-specific fragment yields show that ubiquitin's solution fold is overall unstable in the gas phase, but unfolding caused by loss of solvent is slowest in regions stabilized by salt bridges. ...[more]

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