Identification of cellular genes showing differential expression associated with hepatitis B virus infection.

ABSTRACT:

Aim

To investigate the impact of hepatitis B virus (HBV) infection on cellular gene expression, by conducting both in vitro and in vivo studies.

Methods

Knockdown of HBV was targeted by stable expression of short hairpin RNA (shRNA) in huH-1 cells. Cellular gene expression was compared using a human 30K cDNA microarray in the cells and quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) (qRT-PCR) in the cells, hepatocellular carcinoma (HCC) and surrounding non-cancerous liver tissues (SL).

Results

The expressions of HBsAg and HBx protein were markedly suppressed in the cells and in HBx transgenic mouse liver, respectively, after introduction of shRNA. Of the 30K genes studied, 135 and 103 genes were identified as being down- and up-regulat

SUBMITTER: Fukuhara Y 

PROVIDER: S-EPMC3345538 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature