Silencing of P2Y(2) receptors reduces intraocular pressure in New Zealand rabbits.

ABSTRACT:

Background and purpose

P2 receptors are involved in the regulation of ocular physiological processes like intraocular pressure (IOP). In the present study, the involvement of P2Y(2) receptors in the hypertensive effect of nucleotides was investigated by use of antagonists and of a siRNA designed for the P2Y(2) receptor.

Experimental approach

Agonists of the P2Y(2) receptor a as well as P2 antagonists were applied to eyes of New Zealand rabbits, and the changes in IOP were followed for up to 6 h. Cloning of the P2Y(2) receptor cDNA was done using a combination of degenerate reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). siRNA was synthesized and tested by immunohistochemistry.

Key results

Single doses of 2-thioUTP, UTP-γ-S and UTP i

SUBMITTER: Martin-Gil A 

PROVIDER: S-EPMC3346251 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature