Functional analysis of the degradation of cellulosic substrates by a Chaetomium globosum endophytic isolate.

ABSTRACT: Most photosynthetically fixed carbon is contained in cell wall polymers present in plant biomasses, the largest organic carbon source in the biosphere. The degradation of these polymers for biotechnological purposes requires the combined action of several enzymes. To identify new activities, we examined which enzymes are activated by an endophytic strain of Chaetomium globosum to degrade cellulose-containing substrates. After biochemical analyses of the secretome of the fungus grown on cellulose or woody substrates, we took advantage of the available genomic data to identify potentially involved genes. After in silico identification of putative genes encoding either proteins able to bind to cellulose or glycohydrolases (GHs) of family 7, we investigated their transcript levels by reverse t

SUBMITTER: Longoni P 

PROVIDER: S-EPMC3346360 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature