Community structure analysis of methanogens associated with rumen protozoa reveals bias in universal archaeal primers.

ABSTRACT: The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification.

SUBMITTER: Tymensen LD 

PROVIDER: S-EPMC3346394 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature