Genome-wide alterations in gene methylation by the BRAF V600E mutation in papillary thyroid cancer cells.

ABSTRACT: The BRAF V600E mutation plays an important role in the tumorigenesis of papillary thyroid cancer (PTC). To explore an epigenetic mechanism involved in this process, we performed a genome-wide DNA methylation analysis using a methylated CpG island amplification (MCA)/CpG island microarray system to examine gene methylation alterations after shRNA knockdown of BRAF V600E in thyroid cancer cells. Our results revealed numerous methylation targets of BRAF V600E mutation with a large cohort of hyper- or hypo-methylated genes in thyroid cancer cells, which are known to have important metabolic and cellular functions. As hypomethylation of numerous genes by BRAF V600E was particularly a striking finding, we took a further step to examine the selected 59 genes that became hypermethylated in both ce

SUBMITTER: Hou P 

PROVIDER: S-EPMC3346957 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature