Rapid and sensitive detection of Mycobacterium ulcerans by use of a loop-mediated isothermal amplification test.

ABSTRACT: This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions.

SUBMITTER: Njiru ZK 

PROVIDER: S-EPMC3347114 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature