Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools.

ABSTRACT: A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.

SUBMITTER: Zhang W 

PROVIDER: S-EPMC3347122 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature