Lentiviral vector integration in the human genome induces alternative splicing and generates aberrant transcripts.

ABSTRACT: Retroviral vectors integrate in genes and regulatory elements and may cause transcriptional deregulation of gene expression in target cells. Integration into transcribed genes also has the potential to deregulate gene expression at the posttranscriptional level by interfering with splicing and polyadenylation of primary transcripts. To examine the impact of retroviral vector integration on transcript splicing, we transduced primary human cells or cultured cells with HIV-derived vectors carrying a reporter gene or a human β-globin gene under the control of a reduced-size locus-control region (LCR). Cells were randomly cloned and integration sites were determined in individual clones. We identified aberrantly spliced, chimeric transcripts in more than half of the targeted genes in all cell t

SUBMITTER: Moiani A 

PROVIDER: S-EPMC3347495 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature