Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells.

ABSTRACT:

Background

Control of translation allows for rapid adaptation of the cell to stimuli, rather than the slower transcriptional control. We presume that translational control is an essential process in the control of adipogenesis, especially in the first hours after hormonal stimulation. 3T3-L1 preadipocytes were cultured to confluency and adipogenesis was induced by standard protocols using a hormonal cocktail. Cells were harvested before and 6 hours after hormonal induction. mRNAs attached to ribosomes (polysomal mRNAs) were separated from unbound mRNAs by velocity sedimentation. Pools of polysomal and unbound mRNA fractions were analyzed by microarray analysis. Changes in relative abundance in unbound and polysomal mRNA pools were calculated to detect putative changes in translatio

SUBMITTER: Fromm-Dornieden C 

PROVIDER: S-EPMC3347988 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature