Aurora B and 14-3-3 coordinately regulate clustering of centralspindlin during cytokinesis.

ABSTRACT: Centralspindlin is essential for the formation of microtubule bundle structures and the equatorial recruitment of factors critical for cytokinesis. Stable accumulation of centralspindlin at the spindle midzone requires its multimerization into clusters and Aurora B kinase activity, which peaks at the central spindle during anaphase. Although Aurora B phosphorylates centralspindlin directly, how this regulates centralspindlin localization is unknown. Here we identify a novel regulatory mechanism by which Aurora B enables centralspindlin to accumulate stably at the spindle midzone. We show that 14-3-3 protein binds centralspindlin when the kinesin-6 component MKLP1 is phosphorylated at S710. 14-3-3 prevents centralspindlin from clustering in vitro, and an MKLP1 mutant that is unable to bind

SUBMITTER: Douglas ME 

PROVIDER: S-EPMC3348768 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature