CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription.

ABSTRACT: DNA methylation may regulate gene expression by restricting the access of transcription factors. We have previously demonstrated that GATA-1 regulates the transcription of the CCR3 gene by dynamically interacting with both positively and negatively acting GATA elements of high affinity binding in the proximal promoter region including exon 1. Exon 1 has three CpG sites, two of which are positioned at the negatively acting GATA elements. We hypothesized that the methylation of these two CpGs sites might preclude GATA-1 binding to the negatively acting GATA elements and, as a result, increase the availability of GATA-1 to the positively acting GATA element, thereby contributing to an increase in GATA-1-mediated transcription of the gene. To this end, we determined the methylation of the thre

SUBMITTER: Uhm TG 

PROVIDER: S-EPMC3349909 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature