Evaluation of candidate reference genes for gene expression normalization in Brassica juncea using real time quantitative RT-PCR.

ABSTRACT: The real time quantitative reverse transcription PCR (qRT-PCR) is becoming increasingly important to gain insight into function of genes. Given the increased sensitivity, ease and reproducibility of qRT-PCR, the requirement of suitable reference genes for normalization has become important and stringent. It is now known that the expression of internal control genes in living organism vary considerably during developmental stages and under different experimental conditions. For economically important Brassica crops, only a couple of reference genes are reported till date. In this study, expression stability of 12 candidate reference genes including ACT2, ELFA, GAPDH, TUA, UBQ9 (traditional housekeeping genes), ACP, CAC, SNF, TIPS-41, TMD, TSB and ZNF (new candidate reference genes), in a di

SUBMITTER: Chandna R 

PROVIDER: S-EPMC3350508 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature