EB1-recruited microtubule +TIP complexes coordinate protrusion dynamics during 3D epithelial remodeling.

ABSTRACT:

Background

Epithelial remodeling, in which apical-basal polarized cells switch to a migratory phenotype, plays a central role in development and disease of multicellular organisms. Although dynamic microtubules (MTs) are required for directed migration on flat surfaces, how MT dynamics are controlled or contribute to epithelial remodeling in a more physiological three-dimensional (3D) environment is not understood. We use confocal live-cell imaging to analyze MT function and dynamics during 3D epithelial morphogenesis and remodeling of polarized Madin-Darby canine kidney epithelial cells that undergo partial epithelial-to-mesenchymal transition in response to hepatocyte growth factor (HGF).

Results

We find that HGF treatment increases MT growth rate before morphological chan

SUBMITTER: Gierke S 

PROVIDER: S-EPMC3350573 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature