P2Y1 receptor activation by photolysis of caged ATP enhances neuronal network activity in the developing olfactory bulb.

ABSTRACT: It has recently been shown that adenosine-5'-triphosphate (ATP) is released together with glutamate from sensory axons in the olfactory bulb, where it stimulates calcium signaling in glial cells, while responses in identified neurons to ATP have not been recorded in the olfactory bulb yet. We used photolysis of caged ATP to elicit a rapid rise in ATP and measured whole-cell current responses in mitral cells, the output neurons of the olfactory bulb, in acute mouse brain slices. Wide-field photolysis of caged ATP evoked an increase in synaptic inputs in mitral cells, indicating an ATP-dependent increase in network activity. The increase in synaptic activity was accompanied by calcium transients in the dendritic tuft of the mitral cell, as measured by confocal calcium imaging. The stimulatin

SUBMITTER: Fischer T 

PROVIDER: S-EPMC3350580 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature