Adenosine A1 receptor regulates osteoclast formation by altering TRAF6/TAK1 signaling.

ABSTRACT: Adenosine is an endogenous nucleoside that modulates many physiological processes through four receptor subtypes (A(1), A(2a), A(2b), A(3)). Previous work from our laboratory has uncovered a critical role for adenosine A(1) receptor (A(1) R) in osteoclastogenesis both in vivo and in vitro. Our current work focuses on understanding the details of how A(1) R modulates the receptor activator of NF-κB ligand (RANKL)-induced signaling in osteoclastogenesis. Osteoclasts were generated from mouse bone marrow precursors in the presence of RANKL and macrophage-colony stimulating factor. A pharmacological antagonist of A(1) R (DPCPX) inhibited RANKL-induced osteoclast differentiation, including osteoclast-specific genes (Acp5, MMP9, β(3) Integrin, α(v) Integrin, and CTSK) and osteoclast-specific tra

SUBMITTER: He W 

PROVIDER: S-EPMC3350593 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature