Mutation in the platelet-derived growth factor receptor alpha inhibits adeno-associated virus type 5 transduction.

ABSTRACT: Due to its non-pathogenic lifecycle, little is known about the cellular determinants of infection by adeno-associated virus (AAV). To identify these critical cellular factors, we took advantage of the gene transfer abilities of AAV in combination with a forward genetic selection to identify proteins critical for transduction by this virus. AAV serotype 5 (AAV5) vectors encoding the furin gene were used to transduce furin-deficient cells followed by selection with furin-dependent toxins. A population of cells specifically resistant to AAV5 transduction was identified and sequence analysis suggested all had a single amino acid mutation in the leader sequence of the platelet-derived growth factor receptor alpha (PDGFRα) gene. Characterization of this mutation suggested it inhibited PDGFRα tra

SUBMITTER: Pilz IH 

PROVIDER: S-EPMC3350617 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature