Genome-wide occupancy links Hoxa2 to Wnt-β-catenin signaling in mouse embryonic development.

ABSTRACT: The regulation of gene expression is central to developmental programs and largely depends on the binding of sequence-specific transcription factors with cis-regulatory elements in the genome. Hox transcription factors specify the spatial coordinates of the body axis in all animals with bilateral symmetry, but a detailed knowledge of their molecular function in instructing cell fates is lacking. Here, we used chromatin immunoprecipitation with massively parallel sequencing (ChIP-seq) to identify Hoxa2 genomic locations in a time and space when it is actively instructing embryonic development in mouse. Our data reveals that Hoxa2 has large genome coverage and potentially regulates thousands of genes. Sequence analysis of Hoxa2-bound regions identifies high occurrence of two main classes of

SUBMITTER: Donaldson IJ 

PROVIDER: S-EPMC3351182 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature