Mapping ultra-weak protein-protein interactions between heme transporters of Staphylococcus aureus.

ABSTRACT: Iron is an essential nutrient for the proliferation of Staphylococcus aureus during bacterial infections. The iron-regulated surface determinant (Isd) system of S. aureus transports and metabolizes iron porphyrin (heme) captured from the host organism. Transportation of heme across the thick cell wall of this bacterium requires multiple relay points. The mechanism by which heme is physically transferred between Isd transporters is largely unknown because of the transient nature of the interactions involved. Herein, we show that the IsdC transporter not only passes heme ligand to another class of Isd transporter, as previously known, but can also perform self-transfer reactions. IsdA shows a similar ability. A genetically encoded photoreactive probe was used to survey the regions of IsdC in

SUBMITTER: Abe R 

PROVIDER: S-EPMC3351326 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature