Characterization of DNA primase complex isolated from the archaeon, Thermococcus kodakaraensis.

ABSTRACT: In most organisms, DNA replication is initiated by DNA primases, which synthesize primers that are elongated by DNA polymerases. In this study, we describe the isolation and biochemical characterization of the DNA primase complex and its subunits from the archaeon Thermococcus kodakaraensis. The T. kodakaraensis DNA primase complex is a heterodimer containing stoichiometric levels of the p41 and p46 subunits. The catalytic activity of the complex resides within the p41 subunit. We show that the complex supports both DNA and RNA synthesis, whereas the p41 subunit alone marginally produces RNA and synthesizes DNA chains that are longer than those formed by the complex. We report that the T. kodakaraensis primase complex preferentially interacts with dNTP rather than ribonucleoside triphospha

SUBMITTER: Chemnitz Galal W 

PROVIDER: S-EPMC3351342 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature