Plasticity of adult human pancreatic duct cells by neurogenin3-mediated reprogramming.

ABSTRACT:

Aims/hypothesis

Duct cells isolated from adult human pancreas can be reprogrammed to express islet beta cell genes by adenoviral transduction of the developmental transcription factor neurogenin3 (Ngn3). In this study we aimed to fully characterize the extent of this reprogramming and intended to improve it.

Methods

The extent of the Ngn3-mediated duct-to-endocrine cell reprogramming was measured employing genome wide mRNA profiling. By modulation of the Delta-Notch signaling or addition of pancreatic endocrine transcription factors Myt1, MafA and Pdx1 we intended to improve the reprogramming.

Results

Ngn3 stimulates duct cells to express a focused set of genes that are characteristic for islet endocrine cells and/or neural tissues. This neuro-endocrine shift however,

SUBMITTER: Swales N 

PROVIDER: S-EPMC3351393 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature